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Affinity Chromatography and Chiral Separation LC Columns

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If you are interested in this product, you can Product Name: Affinity Chromatography and Chiral Separation LC Columns
Product Model: AFpak ACH-494 Choline oxydase, acetylcholine este
Product exhibitors: Shodex
Brief:

Affinity Chromatography Affinity chromatography is a well-known method for separating and purifying biological materials, based on their biochemical affinity. The Shodex AFpak series columns use rigid



Affinity Chromatography and Chiral Separation LC ColumnsDetailed description of

The columns have the following features:

1) Rigid polymeric media enable high-performance analysis.

2) Chemically stable bonding groups (spacers) between ligands and substrates minimize detachment of ligands, ensuring highly reproducible analysis. Up to hundreds of runs are reproducible.

 

Product

Ligand

Particle size

Size

Part number

 

 

(micron)

(mm) ID x Length

 

AFpak ACH-494

Choline oxydase, acetylcholine esterase

18

4.6 x 10

F7118964

AFpak ALC-894

Lentil lectin

18

8.0 x 50

F7113080

AFpak ALS-894

Lysine (LCA)

18

8.0 x 50

F7118962

AFpak ANA-894

NAD

18

8.0 x 50

F7113020

AFpak AOV-894

Ovomucoid

18

8.0 x 50

F7118958

AFpak APA-894

Protein A

18

8.0 x 50

F7118946

AFpak APB-894

Aminophenyl boronic acid

10

8.0 x 50

F7118950

AFpak APD-894

Procion red

18

8.0 x 50

F7113030

AFpak APE-894

Phosphorylethanolamine

18

8.0 x 50

F7113070

AFpak APG-894

Protein G

18

8.0 x 50

F7113050

 

Chiral Separation (ORpak CD)

Besides the positive and negative properties of optical rotation, optical isomers have the same physical and chemical characteristics. Optical separation columns are indispensable for direct analysis without derivatization. Shodex ORpak series incorporate packing material bonded by ligands that can identify many optical isomers.

ORpak series has the following ligands: cyclodextrin derivative (CD).

Because CD is used as the ligand, these columns are suitable for the analysis of optical isomers that make up an inclusion complex with CD. alpha-CD is used as the ligand in CDA, beta-CD is used as the ligand in CDB and CDBS and gamma-CD is used as the ligand in CDC. As a result, different columns can be used based on the size of the hydrophobic area of the optical isomer that is included. Packing materials in CDB and CDBS columns are different. Since CDBS is packed with silica gel, the peaks are sharp and many optical isomers can be analysed. Since CDB is packed with polymer-based gel, a wide range of separation conditions is possible, including alkaline separation. Since beta-CD identifies the most optical isomers within the CD's, CDB and CDBS are suitable for preliminary studies of optical isomer analysis. The CD series are suitable for the analysis of, not only optical isomers, but also structural isomers that have similar structure.

Product

Ligand

Particle size

Size

Part number

 

 

(micron)

(mm) ID x Length

 

ORpak CDA-453 HQ

alpha-Cyclodextrin derivatives

6

4.6 x 150

F7146000

ORpak CDB-453 HQ

beta-Cyclodextrin derivatives

6

4.6 x 150

F7146001

ORpak CDC-453 HQ

gamma-Cyclodextrin derivatives

6

4.6 x 150

F7146002

ORpak CDBS-453

beta-Cyclodextrin derivatives

3

4.6 x 150

F7146003

ORpak CD-G

 

6

4.6 x 10

F6709250

 

Chiral Separation (ORpak CRX-853)

These are columns for separating optical isomers by ligand exchange using L-amino acid derivatives as the ligand. Eluent containing copper ions are used such as copper sulfate solutions. These columns can be used to analyze optical isomers of compounds which comprise a complex with copper, such as amino acids and acid hydroxyl groups, as well as their derivatives.

 

Product

Ligand

Particle size

Size

Part number

 

 

(micron)

(mm) ID x Length

 

ORpak CRX-853

L-amino acid derivative

10

8.0 x 50

F7140040

ORpak CRX-G

 

6

4.6 x 10

F6709300

 

Chiral Separation (AFpak ABA-894)

Product

Ligand

Particle size

Size

Part number

 

 

(micron)

(mm) ID x Length

 

AFpak ABA-894

Bovine serum albumin (BSA)

6

8.0 x 50

F7118960


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