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IonOpticks Aurora Frontier XT C18 column 60cmx75μm 1.7 μm
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Product Name:
IonOpticks Aurora Frontier XT C18 column 60cmx75μm 1.7 μm
Product Model:
AUR4-60075C18
Product exhibitors:
IonOpticks
Brief:
IonOpticks Aurora Frontier XT C18 column 60cmx75μm 1.7 μmDetailed description of
Product Overview
The IonOpticks Aurora column (AUR4-60075C18-XT) is a 60 cm × 75 μm nanoflow UHPLC column packed with 1.7 μm C18 (120 Å) particles. It is the first column capable of near‑total proteome coverage. Fully compatible with UHPLC systems (>1700 bar).
Key Specifications
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Dimensions: 60 cm × 75 μm ID
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Phase: Reversed‑phase C18, 1.7 μm, 120 Å
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Max pressure: >1700 bar | Max temp: 60°C
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Flow rate: 100–300 nL/min | Gradient: 90–180 min
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Samples/day: 5–15 | Max load: 1 μg per gradient hour
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Ion sources: EASY‑Spray, Nanospray Flex, Newomics UniESI/DuoESI
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Packaging: 1 column/pack | Origin: Australia
Why Choose This Column?
1. Unmatched proteome coverage – Peak widths: 3.0 s (30 min) / 5.5 s (60 min). Single injection identifies >10,000 HeLa proteins.
2. Zero dead volume – Integrated packed emitter (no post‑column dead volume) + nanoZero® fitting (zero pre‑column dead volume) eliminates band broadening.
3. Built‑in HeatSync™ – Precise column temperature control ensures highly reproducible retention times across hundreds of injections.
4. Exceptional robustness – Maintains performance over >600 consecutive injections, ideal for large cohort studies.
5. Plug‑and‑play – No tools required; hand‑tighten the nanoViper fitting for instant zero‑dead‑volume connection.
Typical Applications
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Ultra‑deep proteomics – 90–180 min gradients, >10,000 proteins/shot.
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PTM analysis – High peak capacity separates low‑abundance modified peptides.
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Single‑cell proteomics – Excellent performance at low‑nanogram loading.
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Large‑scale cohort screening – Stable over hundreds of injections.
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Biopharmaceutical characterization – Peptide mapping of mAbs and subunits.
Maintenance
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Installation: Slide column into source, hand‑tighten 10‑32 fitting.
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Flushing: After each run, flush with 90% ACN/H₂O at 100–300 nL/min for 15–30 min.
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Storage: Seal ends and store in pure ACN or MeOH at 2–8°C.
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Starter pack: AUR4-60075C18-XT-STPK (2 columns + HeatSync™ heater + controller + housing).
Series Comparison
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Aurora Frontier XT (this) – 60 cm × 75 μm, HeatSync™, >1,700 bar, deepest coverage.
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Aurora Ultimate XT – 25 cm × 75/150 μm, balanced resolution & throughput.
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Aurora Elite – 15 cm × 75/150 μm, fast analysis.
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Aurora Rapid – 8 cm × 75/150 μm, ultra‑fast separations.
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