Product Overview
The AUR4‑60075C18‑XT chromatography column is a Generation 4 Aurora® Frontier™ XT nanoflow UHPLC packed emitter from IonOpticks. This AUR4‑60075C18‑XT chromatography column measures 60 cm in length with a 75 μm inner diameter and is packed with 1.7 μm reversed‑phase C18 particles (120 Å pore size). Built‑in HeatSync™ technology provides precise thermal management, delivering the exceptional separation performance required for deep proteome profiling. This AUR4‑60075C18‑XT chromatography column is compatible with EASY‑Spray, Nanospray Flex, and Newomics UniESI/DuoESI ion sources.
Key Specifications
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Dimensions: 60 cm length × 75 μm ID
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Particle size / phase: 1.7 μm / C18, 120 Å pores
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Max pressure: >1700 bar
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Recommended flow rate: 100–300 nL/min
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Recommended gradients: 90–180 min
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Samples per day: 5–15 samples
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Max loading capacity: 1 μg per gradient hour
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pH stability range: 1–8
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Temperature limit: 60℃
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Ion source compatibility: EASY‑Spray, Nanospray Flex, Newomics UniESI, Newomics DuoESI
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Packaging: 1 column per pack
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Origin: Australia
Why Choose This AUR4‑60075C18‑XT Chromatography Column?
1. Patented nanoZero® Zero‑Dead‑Volume Technology
The AUR4‑60075C18‑XT chromatography column features IonOpticks‘ patented nanoZero® fitting, which provides true zero pre‑column dead volume and an integrated packed emitter that eliminates post‑column dead volume. This engineering eliminates band broadening at its source, enabling the highest possible resolution and narrowest peak shapes.
2. Built‑in HeatSync™ for Unmatched Reproducibility
XT‑series columns incorporate HeatSync™ built‑in thermal management, eliminating temperature fluctuations to ensure highly reproducible retention times even across hundreds of injections. This AUR4‑60075C18‑XT chromatography column is an ideal choice for large‑scale cohort studies requiring consistent performance over long sequences.
3. Ultra‑High Peak Capacity
The AUR4‑60075C18‑XT chromatography column delivers class‑leading peak widths: 3.0 seconds FWHM on a 30‑minute gradient and 5.5 seconds on a 60‑minute gradient. This resolution translates directly into deeper proteome coverage and more accurate quantification.
4. Deepest Proteome Coverage
This AUR4‑60075C18‑XT chromatography column is the world‘s first nanoflow column capable of near‑total proteome coverage. HeLa tryptic digest (200 ng) runs on an Orbitrap Exploris 480 with this AUR4‑60075C18‑XT chromatography column identified over 50,000 unique peptides in a 30‑minute gradient and over 77,000 unique peptides in a 60‑minute gradient.
5. Plug‑and‑Play Simplicity
Pre‑fitted with a nanoZero female union that directly accepts a nanoViper fingertight fitting, installation of the AUR4‑60075C18‑XT chromatography column requires no tools—simply slide the column into the ion source and hand‑tighten the 10‑32 fitting for an instant zero‑dead‑volume connection.
Typical Applications
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Ultra‑deep proteomics – The AUR4‑60075C18‑XT chromatography column with 90–180 min gradients on an Orbitrap Exploris 480 delivers >10,000 protein groups and >77,000 unique peptides from a HeLa digest, enabling near‑total proteome coverage.
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In‑depth PTM analysis – The ultra‑high peak capacity of this AUR4‑60075C18‑XT chromatography column effectively separates low‑abundance modified peptides from unmodified ones.
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Single‑cell proteomics – The 75 μm ID format maintains exceptional separation efficiency at low‑nanogram loading levels.
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Large‑scale clinical cohort screening – HeatSync™ built‑in thermal management and outstanding column robustness make this AUR4‑60075C18‑XT chromatography column ideal for reproducible long‑term cohort studies.
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Biopharmaceutical characterization – High‑resolution peptide mapping of monoclonal antibodies and their subunits is another key application of this AUR4‑60075C18‑XT chromatography column.
Maintenance Recommendations
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Installation: Use the nanoZero plug‑and‑play design—slide the AUR4‑60075C18‑XT chromatography column into the ion source and hand‑tighten the 10‑32 fitting. No tools required.
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Column flushing: After each analytical run, flush the AUR4‑60075C18‑XT chromatography column with 90% acetonitrile/water at 100–300 nL/min for 15–30 minutes.
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Storage: For long‑term storage, seal both ends of the AUR4‑60075C18‑XT chromatography column and store in pure acetonitrile or methanol at 2–8°C.
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Starter pack: First‑time users are recommended to purchase the Aurora Frontier™ 60×75 XT C18 HeatSync™ Starter Pack (AUR4‑60075C18‑XT‑STPK).
Series Comparison
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Aurora Frontier XT (This column) – 60 cm × 75 μm, built‑in HeatSync, deepest proteome coverage, >1,700 bar
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Aurora Ultimate – 25 cm × 75/150 μm, balances resolution and throughput for routine proteomics
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Aurora Elite – 15 cm × 75/150 μm, shorter column for faster analysis, suitable for high‑throughput screening
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Aurora Rapid – 8 cm × 75/150 μm, ultra‑fast separations, ideal for method development and rapid screening
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