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IonOpticks Aurora Ultimate CSI C18 column 25cm×75μm, 1.7μm
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Product Name:
IonOpticks Aurora Ultimate CSI C18 column 25cm×75μm, 1.7μm
Product Model:
AUR4-25075C18-CSI
Product exhibitors:
IonOpticks
Brief:
IonOpticks Aurora Ultimate CSI C18 column 25cm×75μm, 1.7μmDetailed description of
Product Overview
The AUR4-25075C18-CSI is IonOpticks' 4th Generation Aurora Ultimate™ CSI C18 UHPLC column. This AUR4-25075C18-CSI column measures 25 cm in length with 75 μm inner diameter, packed with 1.7 μm C18 particles (120 Å pore size), and features a CaptiveSpray Insert (CSI) for seamless integration with Bruker CaptiveSpray, CaptiveSpray 2, and CaptiveSpray Ultra ion sources[reference:0]. The AUR4-25075C18-CSI delivers an ideal balance between separation speed and proteome depth for 45–120 min gradients, processing 10–40 samples per day[reference:1].
Key Specifications
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Product Number: AUR4-25075C18-CSI
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Dimensions: 25 cm × 75 μm ID
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Particle Size / Phase: 1.7 μm C18, 120 Å
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Max Pressure: >1700 bar
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Recommended Flow Rate: 100–400 nL/min
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Recommended Gradient: 45–120 min
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Sample Throughput: 10–40 samples/day
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Max Loading Capacity: 1 μg
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pH Stability: 1–8 | Temperature Limit: 60°C
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Ion Source Compatibility: Bruker CaptiveSpray, CS2, Ultra
Why Choose the AUR4-25075C18-CSI Column?
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CSI Interface for Bruker CaptiveSpray: The AUR4-25075C18-CSI integrates a CaptiveSpray Insert, eliminating the need for adapters when connecting to Bruker CaptiveSpray series ion sources, maximizing ionization efficiency and sensitivity.
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nanoZero® Zero Dead Volume: Patented nanoZero® technology provides a true zero pre-column dead volume connection. The packed emitter design eliminates post-column dead volume, delivering ultra-low peak tailing and the highest chromatographic resolution[reference:2].
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Balance of Throughput & Depth: The AUR4-25075C18-CSI processes 10–40 samples per day with 45–120 min gradients while achieving deep proteome coverage. On a Bruker timsTOF Pro with dia-PASEF, 200 ng HeLa tryptic digest yields thousands of unique protein identifications with average FWHM below 20 seconds[reference:3].
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Plug-and-Play Installation: Pre-fitted with nanoZero® interface (10-32 thread) — hand-tighten only, no tools required.
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Excellent Quantitative Accuracy: The AUR4-25075C18-CSI demonstrates outstanding spike-recovery and reproducibility in SILAC, TMT, and label-free quantification workflows, as validated by yeast spike-in experiments at different ratios[reference:4].
Typical Applications
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Deep Proteomics: Ideal for single-run deep analysis with 45–120 min gradients on timsTOF Pro with dia-PASEF or Slice-PASEF acquisition.
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Single-Cell Proteomics: The 75 μm ID and nano-flow operation enable the AUR4-25075C18-CSI to maintain high efficiency at low-nanogram loading levels, making it ideal for single-cell and low-input proteomics.
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Quantitative Proteomics: Excellent precision for SILAC, TMT, and label-free quantification workflows.
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Clinical Cohort Screening: Robust construction ensures long-term, reproducible runs for large-scale clinical studies.
Peer-Reviewed Publications Featuring AUR4-25075C18-CSI
The AUR4-25075C18-CSI has been cited in numerous high-impact studies across leading journals, demonstrating its reliability and performance in cutting-edge proteomics research:
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Mercer et al. (2024) — Presented a novel approach to overcome limitations in proteomics workflows using the AUR4-25075C18-CSI column[reference:5].
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Kozicka et al. (2024) — Published in Nature Chemical Biology, this study introduced a deep learning‑based chemoproteomics approach leveraging an Aurora Ultimate CSI C18 column[reference:6].
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Qiu et al. (2024) — Published in Cell, this research unveiled a critical role for chromatin regulators using an Aurora Ultimate 25cm×75μm C18 column[reference:7].
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Leduc et al. (2024) — Developed nPOP, a revolutionary method for single‑cell proteomics, utilizing the AUR4-25075C18-CSI column[reference:8].
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Sinn et al. (2025) — Developed Slice-PASEF acquisition on a timsTOF SCP coupled to an Aurora Ultimate™ 25cm×75μm CSI C18 UHPLC column, identifying 84% more precursors and 46% more protein groups from 10 ng samples[reference:9].
Usage & Maintenance
Installation: Hand-tighten the 10-32 fitting — no tools required. Washing: After each run, flush the AUR4-25075C18-CSI with 90% acetonitrile/water at 100–400 nL/min for 15–30 min. Storage: Seal ends and store in pure acetonitrile or methanol at 2–8°C.
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