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IonOpticks Aurora Ultimate CSI C18 column 1.7μm 25cm×75μm
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Product Name:
IonOpticks Aurora Ultimate CSI C18 column 1.7μm 25cm×75μm
Product Model:
AUR4-25075C18
Product exhibitors:
IonOpticks
Brief:
IonOpticks Aurora Ultimate CSI C18 column 1.7μm 25cm×75μmDetailed description of
Overview
The AUR4-25075C18 is IonOpticks' 4th‑generation Aurora Ultimate™ nanoUHPLC column. This AUR4-25075C18 column measures 25 cm length × 75 μm ID, packed with 1.7 μm C18 (120 Å pore), and features a CSI (CaptiveSpray Insert) interface for Bruker CaptiveSpray ion sources. The AUR4-25075C18 delivers an ideal balance between separation speed and proteome depth for 45–120 min gradients.
Key Specifications
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Part Number: AUR4-25075C18
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Dimensions: 25 cm × 75 μm
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Particle/Phase: 1.7 μm C18, 120 Å
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Max Pressure: >1700 bar
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Flow Rate: 100–400 nL/min
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Recommended Gradient: 45–120 min
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Sample Throughput: 10–40 samples/day
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Max Loading: 1 μg
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pH Range: 1–8 | Temp Limit: 60 °C
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Ion Source Compatibility: Bruker CaptiveSpray, CS2, Ultra
Why Choose the AUR4-25075C18 Column?
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CSI Interface for Bruker: The AUR4-25075C18 integrates CaptiveSpray Insert (CSI) – no adapters needed for Bruker CaptiveSpray sources, maximizing ionization efficiency.
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nanoZero® Zero Dead Volume: Patented technology eliminates pre‑column and post‑column dead volume, delivering ultra‑low peak tailing and highest resolution.
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Balance of Throughput & Depth: The AUR4-25075C18 column processes 10–40 samples/day (45–120 min gradients) while achieving deep proteome coverage. On a Bruker timsTOF Pro with dia‑PASEF, 200 ng HeLa digest yields thousands of protein IDs.
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Plug‑and‑Play: Pre‑fitted nanoZero interface (10‑32 thread) – hand‑tighten only, no tools required.
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Excellent Quantitative Accuracy: The AUR4-25075C18 shows outstanding spike‑recovery and reproducibility in SILAC, TMT, and label‑free workflows.
Typical Applications
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Deep Proteomics: Ideal for single‑run deep analysis with 45–120 min gradients, especially on timsTOF Pro with dia‑PASEF.
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Single‑Cell Proteomics: 75 μm ID and nano‑flow operation – the AUR4-25075C18 maintains high efficiency at low‑nanogram loading.
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Quantitative Proteomics: Excellent precision for SILAC, TMT, and label‑free quantification.
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Clinical Cohort Screening: Robust construction ensures long‑term, reproducible runs for large clinical studies.
Usage & Maintenance
Installation: Hand‑tighten the 10‑32 fitting – no tools. Washing: After each run, flush the AUR4-25075C18 column with 90% acetonitrile/water at 100–400 nL/min for 15–30 min. Storage: Seal ends and store in pure acetonitrile or methanol at 2‑8 °C.
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