Product Overview
The AUR4‑60075C18‑CSI chromatography column is a 60 cm × 75 μm nanoflow UHPLC packed emitter from IonOpticks‘ Generation 4 Aurora® Frontier™ series. This AUR4‑60075C18‑CSI chromatography column is packed with 1.7 μm reversed‑phase C18 particles (120 Å pores) and is fitted with a dedicated CaptiveSpray Insert (CSI) for seamless integration with Bruker ion sources. It is the world‘s first nanoflow column capable of delivering near‑total proteome coverage.
Key Specifications
The AUR4‑60075C18‑CSI chromatography column delivers the following key specifications:
-
Length × ID: 60 cm × 75 μm
-
Particle size / phase: 1.7 μm / C18
-
Pore size: 120 Å
-
Max pressure: >1700 bar
-
Recommended flow rate: 100–300 nL/min
-
Recommended gradients: 90–180 min
-
Samples per day: 5–15
-
Max loading capacity: 1 μg per gradient hour
-
Ion source compatibility: Bruker CaptiveSpray, CaptiveSpray 2, CaptiveSpray Ultra; with grounding cable HVCABLE02
-
Packaging: 1 column per pack
Why Choose This AUR4‑60075C18‑CSI Chromatography Column?
1. CSI Interface for Bruker CaptiveSpray Systems
This AUR4‑60075C18‑CSI chromatography column is fitted with a CaptiveSpray Insert (CSI) that works directly with Bruker CaptiveSpray, CaptiveSpray 2 and CaptiveSpray Ultra ion sources. With the included HVCABLE02 grounding cable, installation is straightforward and spray stability is assured. The CSI version has become the standard nanoflow separation column for Bruker timsTOF mass spectrometers.
2. NanoZero® Zero‑Dead‑Volume Technology
The patented nanoZero® fitting provides true zero pre‑column dead volume and a fully packed integrated emitter eliminates post‑column dead volume. This AUR4‑60075C18‑CSI chromatography column delivers exceptionally low peak tailing, the highest possible resolution, and plug‑and‑play installation without special tools.
3. Generation 4 Aurora Series® Performance
The AUR4‑60075C18‑CSI chromatography column incorporates multiple Generation 4 innovations—including improved spray stability and enhanced mechanical robustness—that provide a step‑change in performance on long gradients. It is the first column ever to enable near‑full proteome coverage, thanks to its unmatched peak capacity.
4. Ultra‑High Peak Capacity
With this AUR4‑60075C18‑CSI chromatography column, the average peak width is only 3.0 seconds on a 30‑minute gradient and 5.5 seconds on a 60‑minute gradient. This resolution translates directly into deeper proteome coverage and more accurate quantification.
5. Unmatched Robustness
The AUR4‑60075C18‑CSI chromatography column withstands >600 consecutive injections while maintaining high sensitivity, resolution and run‑to‑run stability. It is ideally suited for large‑scale cohort proteomics and other demanding high‑throughput applications.
6. HeatSync™ Ready
The AUR4‑60075C18‑CSI chromatography column is fully compatible with the HeatSync™ temperature control system for precise, stable column heating, ensuring highly reproducible retention times over long sequences.
Typical Applications
-
Ultra‑deep proteomics – On an Orbitrap Exploris 480, the AUR4‑60075C18‑CSI chromatography column has consistently identified >10,000 proteins from HeLa digests.
-
In‑depth PTM analysis – The AUR4‑60075C18‑CSI chromatography column effectively separates low‑abundance phosphorylated/glycosylated peptides from unmodified ones.
-
Single‑cell proteomics – The 75 μm ID format maintains exceptional separation efficiency at low‑nanogram loading levels.
-
Large‑scale cohort screening – The AUR4‑60075C18‑CSI chromatography column delivers stable performance across hundreds of injections without performance drop.
-
Biopharmaceutical characterization – High‑resolution peptide mapping of monoclonal antibodies and subunits.
Operation Recommendations
-
Installation: Slide the AUR4‑60075C18‑CSI chromatography column into the ion source and hand‑tighten the 10‑32 fitting (use HVCABLE02 grounding cable). Installation requires no special tools.
-
Column flushing: After each run, flush with 90% acetonitrile/water at 100–300 nL/min for 15–30 minutes.
-
Storage: For long‑term storage, seal both ends and store in pure acetonitrile or methanol at 2–8°C.
Series Product Comparison
The Generation 4 Aurora Series® family includes several formats to match different application needs:
-
Aurora Frontier CSI (this product): 60 cm × 75 μm, CSI interface, deepest proteome coverage
-
Aurora Ultimate: 25 cm × 75 μm / 150 μm, balances resolution and throughput
-
Aurora Elite: 15 cm × 75 μm / 150 μm, shorter column for faster analysis
-
Aurora Rapid: 8 cm × 75 μm / 150 μm, ultra‑fast separations
Leave a message for product