IonOpticks Aurora Ultimate XT C18 column 25cm×75μm, 1.7μm

Product Overview

TheAUR4-25075C18-XTis IonOpticks' 4th Generation Aurora Ultimate™ XT C18 UHPLC column. ThisAUR4-25075C18-XT columnmeasures 25 cm in length with 75 μm inner diameter, packed with 1.7 μm C18 particles (120 Å pore size), and features built-in HeatSync™ technology. TheAUR4-25075C18-XTis compatible with ****** Scientific EASY-Spray and Nanospray Flex ion sources, as well as Newomics UniESI and DuoESI sources. Engineered for discovery proteomics, theAUR4-25075C18-XTdelivers an ideal balance between separation speed and proteome depth for 45–120 min gradients, processing 10–40 samples per day.[reference:0][reference:1][reference:2]

Key Specifications

• Part Number: AUR4-25075C18-XT
• Dimensions: 25 cm × 75 μm ID
• Particle Size / Phase: 1.7 μm C18, 120 Å
• Max Pressure: >1700 bar
• Recommended Flow Rate: 100–400 nL/min
• Recommended Gradient: 45–120 min
• Sample Throughput: 10–40 samples/day
• Max Loading Capacity: 1 μg
• pH Stability: 1–8 |Temperature Limit: 60°C
• Ion Source Compatibility: EASY-Spray, Nanospray Flex, UniESI, DuoESI

Why Choose the AUR4-25075C18-XT Column?

• Built-in HeatSync™ Technology: TheAUR4-25075C18-XTintegrates HeatSync™ column heating, which maintains optimal column temperature from injection to detection, preserving chromatographic performance and ensuring run-to-run reproducibility.[reference:3]
• Sustainable and Reusable Heating Solution: Unlike systems where the heating element is embedded in the column housing, the XT heater solution is reusable. When you discard spent columns, the unique column heater remains ready for your next experiment, minimizing waste and reducing long-term costs.[reference:4]
• Wide Ion Source Compatibility: TheAUR4-25075C18-XTis compatible with ****** Scientific EASY-Spray and Nanospray Flex, as well as Newomics UniESI and DuoESI ion sources, providing seamless integration across major MS platforms.[reference:5]
• nanoZero® Zero Dead Volume: Patented nanoZero® technology provides true zero pre-column dead volume connections, delivering ultra-low peak tailing and the highest chromatographic resolution.[reference:6]
• Exceptional Performance in Discovery Proteomics: TheAUR4-25075C18-XT columndemonstrates exceptional performance in discovery proteomics applications, with the ability to deliver high-resolution separations and deeper proteome coverage.[reference:7]

Typical Applications

• Deep Proteomics: TheAUR4-25075C18-XTis ideal for single-run deep analysis with 45–120 min gradients, delivering thousands of protein identifications from HeLa digests.
• Single-Cell Proteomics: The 75 μm ID enables high-efficiency separation at low-nanogram loading levels, making theAUR4-25075C18-XTideal for single-cell and limited-sample proteomics.[reference:8]
• Quantitative Proteomics: Excellent precision for SILAC, TMT, and label-free quantification workflows, supported by high reproducibility across runs.
• Clinical Cohort Screening: Robust construction ensures long-term, reproducible operation for large-scale clinical studies.

Peer-Reviewed Publications Featuring AUR4-25075C18-XT

TheAUR4-25075C18-XThas been extensively validated in high-impact proteomics research:

• IonOpticks Application Note (2024)— Combined theAUR4-25075C18-XT columnwith the Q Exactive Plus to elevate the performance of this classic instrument, demonstrating maximum protein and peptide identifications across a broad range of sample loads, gradients, and search algorithms.[reference:9]
• MAP-MS Method Development (2025)— Experiments performed using an Exploris 480 with an EASY-nLC 1200 and theAUR4-25075C18-XT, enabling improved proteomic dynamic range.[reference:10]
• Astral Mass Spectrometer Evaluation (2025)— Peptides separated using a Vanquish Neo UHPLC with theAUR4-25075C18-XT, achieving high-throughput runs with 20–37 min gradients.[reference:11]
• Single-Cell Proteomics Study (2024)— TheAUR4-25075C18-XTenabled unprecedented proteome coverage and accuracy in quantifying ultra-low input amounts in single-cell proteomics.[reference:12]

Usage & Maintenance

Installation: TheAUR4-25075C18-XTfeatures a pre-fitted nanoZero® interface (10-32 thread) — hand-tighten only, no tools required. For EASY-Spray sources, follow the HeatSync™ Controller or IonOpticks Heater Controller installation guide.[reference:13]
Washing: After each run, flush theAUR4-25075C18-XTwith 90% acetonitrile/water at 100–400 nL/min for 15–30 min.
Storage: Seal ends and store in pure acetonitrile or methanol at 2–8°C.

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