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ionopticks Aurora Ultimate XS C18 UHPLC Column 25cm×75μm, 1.7μm C18
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Product Name:
ionopticks Aurora Ultimate XS C18 UHPLC Column 25cm×75μm, 1.7μm C18
Product Model:
AUR4-25075C18-XS
Product exhibitors:
IonOpticks
Brief:
ionopticks Aurora Ultimate XS C18 UHPLC Column 25cm×75μm, 1.7μm C18Detailed description of
Product Overview
TheAUR4-25075C18-XSis IonOpticks' 4th Generation Aurora Ultimate™ XS C18 UHPLC column. ThisAUR4-25075C18-XS columnmeasures 25 cm in length with 75 μm inner diameter, packed with 1.7 μm C18 particles (120 Å pore size), and features built-in HeatSync™ technology. TheAUR4-25075C18-XSis specifically optimized for SCIEX ZenoTOF systems and is fully compatible with SCIEX OptiFlow Pro and OptiFlow Turbo V ion sources. Engineered for high-sensitivity discovery proteomics, theAUR4-25075C18-XSdelivers an ideal balance between separation speed and proteome depth for 45–120 min gradients, processing 10–40 samples per day.
Key Specifications
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Part Number: AUR4-25075C18-XS
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Dimensions: 25 cm × 75 μm ID
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Particle Size / Stationary Phase: 1.7 μm C18, 120 Å
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Max Pressure Rating: >1700 bar
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Recommended Flow Rate: 100–400 nL/min
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Recommended Gradient: 45–120 min
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Sample Throughput: 10–40 samples/day
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Max Loading Capacity: 1 μg
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pH Stability Range: 1–8
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Maximum Temperature: 60°C (with HeatSync™ Column Heater)
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Ion Source Compatibility: SCIEX OptiFlow Pro, OptiFlow Turbo V
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Origin: Australia
Why Choose the AUR4-25075C18-XS Column?
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Built-in HeatSync™ Technology for Precise Thermal Control: TheAUR4-25075C18-XSintegrates HeatSync™ column heating, which maintains optimal column temperature from injection to detection, preserving chromatographic performance and ensuring exceptional run-to-run reproducibility.
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Sustainable, Reusable Heating Solution: The XS heater solution is fully reusable — when you discard spent columns, the unique column heater remains ready for your next experiment, minimizing waste and reducing long-term operating costs.
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Optimized for SCIEX ZenoTOF Systems: TheAUR4-25075C18-XSis uniquely designed to maximize peptide and protein analysis on the ZenoTOF line. The enhanced sensitivity of this combination enables identification and quantitation of proteins from complex samples with unprecedented accuracy and significantly expands the depth of proteome coverage.
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Full Compatibility with SCIEX OptiFlow Ion Sources: TheAUR4-25075C18-XSworks seamlessly with SCIEX OptiFlow Pro and OptiFlow Turbo V ion sources, ensuring straightforward integration into existing SCIEX MS workflows.
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nanoZero® Zero Dead Volume Technology: TheAUR4-25075C18-XS columnfeatures integrated nanoZero® technology, which provides true zero pre-column dead volume connections. The unique packed emitter design also eliminates post-column dead volume, delivering ultra-low peak tailing and the highest chromatographic resolution.
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Exceptional Performance in Deep Proteomics: TheAUR4-25075C18-XSdemonstrates exceptional performance in deep proteomics applications, delivering high-resolution separations with outstanding reproducibility across thousands of injections.
Typical Applications
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Deep Proteomics with ZenoTOF Systems: TheAUR4-25075C18-XSis ideal for single-run deep analysis on SCIEX ZenoTOF 7600 and 7600+ systems, delivering thousands of protein identifications from complex samples with exceptional sensitivity.
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Single-Cell Proteomics: The 75 μm ID enables high-efficiency separation at low-nanogram loading levels, making theAUR4-25075C18-XSideal for single-cell and limited-input proteomics workflows.
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High-Throughput Quantitative Proteomics: TheAUR4-25075C18-XSprocesses 10–40 samples per day with 45–120 min gradients, delivering excellent precision for label-free and multiplexed quantification workflows with high run-to-run reproducibility.
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Clinical Biomarker Discovery: Robust construction ensures long-term, reproducible operation for large-scale clinical cohort studies.
Industry Collaborations & Validation
TheAUR4-25075C18-XShas been extensively validated through IonOpticks' strategic co-marketing agreement with SCIEX, combining the Aurora Series XS range with the ZenoTOF line including the latest ZenoTOF 7600+ system. SCIEX Technical Product Manager Ihor Batruch noted: "I would say these columns are the best on the market. They'll allow you to explore more of the proteome with significant gains in performance, coverage and sensitivity".
TheAUR4-25075C18-XSis widely cited in peer-reviewed proteomics publications and ASMS presentations. At ASMS 2024, a high-throughput single-cell proteomics workflow processing 50 samples per day using an IonOpticks 25 cm Aurora Ultimate column demonstrated the ultra-sharp peaks and exceptional reproducibility characteristic of the Aurora series.
Usage & Maintenance
Installation: TheAUR4-25075C18-XSfeatures a pre-fitted nanoZero® interface (10-32 thread) — hand-tighten only, no tools required. Simply place the IonOpticks Column Heater onto the fin of the Aurora XS column for optimal temperature control.
Washing: After each run, flush theAUR4-25075C18-XSwith 90% acetonitrile/water at 100–400 nL/min for 15–30 min.
Storage: Seal ends and store in pure acetonitrile or methanol at 2–8°C.
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