Product Overview
TheAUR4-15075C18is IonOpticks' 4th Generation Aurora Elite™ C18 UHPLC column. ThisAUR4-15075C18 columnmeasures 15 cm in length with 75 μm inner diameter, packed with 1.7 μm C18 particles (120 Å pore size), and features a pre-fitted nanoZero® interface. TheAUR4-15075C18is fully compatible with Nanospray Flex ion sources and is engineered as an "experiment in a day" solution, delivering the perfect balance between high throughput and depth of coverage for 10–45 minute gradients, processing 30–60 samples per day.
Key Specifications
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Part Number: AUR4-15075C18
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Dimensions: 15 cm × 75 μm ID
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Particle Size / Stationary Phase: 1.7 μm C18, 120 Å
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Max Pressure Rating: >1700 bar
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Recommended Flow Rate: 100–600 nL/min
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Recommended Gradient: 10–45 min
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Sample Throughput: 30–60 samples/day
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Max Loading Capacity: 500 ng
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pH Stability Range: 1–8
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Maximum Temperature: 60°C
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Ion Source Compatibility: Nanospray Flex, EASY-Spray, CaptiveSpray, Newomics UniESI, OptiFlow Turbo V
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Origin: Australia
Why Choose the AUR4-15075C18 Column?
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Ultra-High Throughput for Discovery Proteomics: TheAUR4-15075C18delivers exceptional sample throughput, processing 30–60 samples per day using 10–45 minute gradients. This makes theAUR4-15075C18 columnideal for large-scale cohort studies, clinical screening, and high-demand proteomics facilities.
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nanoZero® Zero Dead Volume Technology: TheAUR4-15075C18features IonOpticks' proprietary nanoZero® fitting, which provides true zero pre-column dead volume connections. The unique packed emitter design also eliminates post-column dead volume, delivering ultra-low peak tailing, the highest chromatographic resolution, and maximum mobile phase velocity.
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Exceptional Balance of Throughput and Coverage: TheAUR4-15075C18is specifically tailored to achieve the deep discovery synonymous with the Aurora Series® columns while enabling researchers to leverage "experiment in a day" workflows. With a 15 cm length, theAUR4-15075C18 columnoffers outstanding proteome coverage for its rate of throughput.
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Robust and Long-Lasting: Designed to withstand ultra-high-performance LC backpressures exceeding 1700 bar, theAUR4-15075C18is built for durability. The conductive nanoZero® fitting allows a voltage connection directly to the column entrance. Field reports indicate that Aurora Series columns last at least five times longer than home-packed columns.
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Plug-and-Play Installation: TheAUR4-15075C18 columnfeatures a pre-fitted nanoZero® interface (10-32 thread) — hand-tighten only, no tools required. The single nano connection makes setup easy and simplifies troubleshooting, saving valuable laboratory time.
Typical Applications
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High-Throughput Proteomics: TheAUR4-15075C18is the ideal choice for laboratories requiring rapid sample turnover. With 10–45 minute gradients and 30–60 samples per day, thisAUR4-15075C18 columnenables deep discovery proteomics at unprecedented speed.
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Clinical Cohort Screening: The robust construction and exceptional reproducibility of theAUR4-15075C18make it suitable for large-scale clinical studies requiring consistent performance across thousands of injections.
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Label-Free Quantification (LFQ): The narrow peak widths and high resolution provided by theAUR4-15075C18 columnmaximize protein identifications in label-free quantitative workflows, delivering superior precision and data completeness.
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Phosphoproteomics and PTM Analysis: TheAUR4-15075C18delivers sharp, well-resolved peaks essential for confident post-translational modification localization and quantification in complex biological samples.
Industry Validation and Citations
TheAUR4-15075C18has been extensively validated by leading proteomics researchers worldwide and is featured in numerous peer-reviewed publications and technical notes. IonOpticks' Generation 4 Aurora Series® columns, including theAUR4-15075C18 column, are the result of extensive collaboration with the global proteomics community.
Researchers have reported that switching from home-packed columns to the Aurora Series significantly improves data quality, spray stability, and durability. As noted by Dr. Alex von Kriegsheim, Edinburgh Cancer Research UK Centre: "We have switched all our instruments from in-house packed 35cm emitters to Aurora Series columns. Their performance is equal or superior to our in-house packed columns, their spray-stability and durability is exceptional, they are fool-proof to install and last hundreds of runs."
TheAUR4-15075C18 columnhas been successfully deployed on major mass spectrometry platforms including ****** Scientific Orbitrap Astral, Orbitrap Exploris, Q Exactive Plus, and Bruker timsTOF systems, demonstrating outstanding reproducibility and narrow peak widths across different instrument configurations.
Usage & Maintenance
Installation: TheAUR4-15075C18features a pre-fitted nanoZero® interface (10-32 thread) — hand-tighten only, no tools required. Refer to the IonOpticks online video guides for detailed installation instructions.
Washing: After each run, flush theAUR4-15075C18 columnwith 90% acetonitrile/water at 100–600 nL/min for 15–30 minutes.
Storage: Seal ends and store in pure acetonitrile or methanol at 2–8°C.
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