IonOpticks Aurora Elite CSI C18 column 15cm×75μm, 1.7μm

Product Overview

The AUR4-15075C18-CSI is IonOpticks' 4th Generation Aurora Elite™ CSI C18 UHPLC column. This AUR4-15075C18-CSI column measures 15 cm in length with 75 μm inner diameter, packed with 1.7 μm C18 particles (120 Å pore size), and features a CaptiveSpray Insert (CSI) for seamless integration with Bruker CaptiveSpray, CaptiveSpray 2, and CaptiveSpray Ultra ion sources. Designed as an "experiment in a day" solution, the AUR4-15075C18-CSI delivers the perfect balance between high throughput and proteome coverage depth for 10–45 minute gradients, processing 30–60 samples per day.[reference:0][reference:1]

Key Specifications

• Part Number: AUR4-15075C18-CSI
• Dimensions: 15 cm × 75 μm ID
• Particle Size / Stationary Phase: 1.7 μm C18, 120 Å
• Max Pressure Rating: >1700 bar
• Recommended Flow Rate: 100–600 nL/min
• Recommended Gradient: 10–45 min
• Sample Throughput: 30–60 samples/day
• Max Loading Capacity: 500 ng
• pH Stability Range: 1–8
• Maximum Temperature: 60°C
• Ion Source Compatibility: Bruker CaptiveSpray, CaptiveSpray 2, CaptiveSpray Ultra
• Origin: Australia

Why Choose the AUR4-15075C18-CSI Column?

• CSI Interface for Bruker CaptiveSpray: The AUR4-15075C18-CSI integrates a CaptiveSpray Insert, enabling direct connection to Bruker CaptiveSpray series ion sources without adapters. This integration maximizes ionization efficiency and sensitivity, making the AUR4-15075C18-CSI column the ideal choice for Bruker timsTOF systems.[reference:2]
• nanoZero® Zero Dead Volume Technology: The AUR4-15075C18-CSI features IonOpticks' proprietary nanoZero® fitting, which provides true zero pre-column dead volume connections. The unique packed emitter design eliminates post-column dead volume, delivering ultra-low peak tailing, the highest chromatographic resolution, and maximum mobile phase velocity.[reference:3][reference:4]
• High Throughput Without Compromising Coverage: The AUR4-15075C18-CSI is specifically tailored to achieve the deep discovery synonymous with Aurora Series® columns while enabling researchers to leverage "experiment in a day" workflows. With 10–45 minute gradients and 30–60 samples per day, the AUR4-15075C18-CSI column offers outstanding proteome coverage for its rate of throughput.[reference:5]
• Exceptional Durability and Spray Stability: Built to withstand backpressures exceeding 1700 bar, the AUR4-15075C18-CSI is designed for long-lasting performance. Field reports indicate that Aurora Series columns last significantly longer than home-packed columns, maintaining spray stability and chromatographic performance across hundreds of injections.[reference:6]
• Plug-and-Play Installation: The AUR4-15075C18-CSI column features a pre-fitted nanoZero® interface (10-32 thread) — hand-tighten only, no tools required. The single nano connection simplifies setup and troubleshooting, saving valuable laboratory time.[reference:7]

Typical Applications

• High-Throughput Proteomics: The AUR4-15075C18-CSI is the ideal choice for laboratories requiring rapid sample turnover. With 10–45 minute gradients and 30–60 samples per day, this AUR4-15075C18-CSI column enables deep discovery proteomics at unprecedented speed.
• Single-Cell Proteomics: The 75 μm ID and nano-flow operation enable the AUR4-15075C18-CSI to maintain high efficiency at low-nanogram loading levels, making it ideal for single-cell and limited-input proteomics workflows. The 15 cm column length has enabled quantification of up to 2,500 proteins from a single HeLa cell.[reference:8]
• Clinical Biomarker Discovery: The robust construction and exceptional reproducibility of the AUR4-15075C18-CSI column make it suitable for large-scale clinical cohort studies requiring consistent performance across thousands of injections.
• Plasma Proteomics and Deep Profiling: The AUR4-15075C18-CSI has been successfully deployed in plasma proteome mining studies. In a quantitative proteomics study published in the Journal of Proteomics (2025), an Evosep One coupled to a timsTOF HT with an Aurora Elite™ 15×75 CSI C18 UHPLC column using the Whisper Zoom 40 SPD method achieved deep plasma proteome coverage.[reference:9]
• T Cell Differentiation and Immune Proteomics: Researchers used an IonOpticks Aurora Elite CSI 15cm column for chromatographic separation in a longitudinal T cell differentiation study, quantifying over 6,600 proteins and enabling critical discoveries in exhaustion biology.[reference:10]

Peer-Reviewed Publications Featuring AUR4-15075C18-CSI

The AUR4-15075C18-CSI has been extensively validated in high-impact proteomics research:

• Roger et al. (2025) — Journal of Proteomics: "Mining the plasma proteome: Evaluation of enrichment methods for depth and reproducibility." The study utilized an Aurora Elite™ 15×75 CSI C18 UHPLC column coupled to a timsTOF HT via an Evosep One, demonstrating deep plasma proteome coverage and superior quantification reproducibility.[reference:11]
• Beusch et al. (2024) — BioRxiv: "Longitudinal proteomic profiling of T cell differentiation in vivo unveils dynamic proteome remodeling." The researchers used an Aurora Elite CSI 15cm column for chromatographic separation, quantifying over 6,600 proteins across the dataset and enabling discoveries in T cell exhaustion biology.[reference:12]
• Single-Cell Proteomics Study: The 15 cm Aurora Elite column has been shown to quantify up to 2,500 proteins from a single HeLa cell, demonstrating its sensitivity and resolution for ultra-low-input sample analysis.[reference:13]
• High-Throughput Proteomics Evaluation: The Aurora Elite 15 cm column has been evaluated for 600+ consecutive injections while maintaining high sensitivity, efficiency, and peak stability, confirming its long-term robustness.[reference:14]

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